Micropatterned avidin arrays on silicon substrates via photolithography, self‐assembly and bioconjugation
- 1 February 2006
- journal article
- Published by Wiley in Biotechnology and Applied Biochemistry
- Vol. 43 (2), 85-91
- https://doi.org/10.1042/ba20050085
Abstract
Processes for micropatterning protein arrays on inorganic substrates have gained attention in the development of biosensors and clinical diagnostics. This study demonstrates a chemically selective method based on photolithographic deposition of gold patterns with the subsequent attachment of functionalized alkanethiols via molecular self-assembly. Selective capping of carboxy groups on alkanethiols by N-hydroxysuccinimide esters allowed the deposition of methoxypoly(ethylene glycol) silane as a blocking agent on unpatterned regions. Carboxylates were used to couple a form of avidin to create a microarray of protein. This microarray was successfully probed with biotinylated quantum dots. In-process characterization methods included grazing-angle Fourier-transform IR spectroscopy, ellipsometry, contact-angle goniometry, atomic-force microscopy and fluorescence microscopy.Keywords
This publication has 20 references indexed in Scilit:
- Development of a High-Throughput Automated Analyzer Using Biochip Array TechnologyClinical Chemistry, 2005
- A New Method of Preparing Monolayers on Silicon and Patterning Silicon Surfaces by Scribing in the Presence of Reactive SpeciesLangmuir, 2001
- Dip-Pen Nanolithography: Automated Fabrication of Custom Multicomponent Sub-100-Nanometer Surface ArchitecturesMRS Bulletin, 2001
- Microfluidic Networks Made of Poly(dimethylsiloxane), Si, and Au Coated with Polyethylene Glycol for Patterning Proteins onto SurfacesLangmuir, 2001
- Influence of Surface Topography on Alkanethiol SAMs Assembled from Solution and by Microcontact PrintingLangmuir, 2001
- Immobilization of Protein Molecules onto Homogeneous and Mixed Carboxylate-Terminated Self-Assembled MonolayersLangmuir, 1997
- Microfluidics-a reviewJournal of Micromechanics and Microengineering, 1993
- Formation of monolayers by the coadsorption of thiols on gold: variation in the length of the alkyl chainJournal of the American Chemical Society, 1989
- Formation of monolayers by the coadsorption of thiols on gold: variation in the head group, tail group, and solventJournal of the American Chemical Society, 1989
- An introduction to the concepts and technology of biosensorsBiosensors, 1985