The precipitation of polyanions by long-chain aliphatic ammonium compounds IV. Elution in salt solutions of mucopolysaccharide-quaternary ammonium complexes adsorbed on a support
- 9 December 1961
- journal article
- Published by Elsevier BV in Biochimica et Biophysica Acta
- Vol. 54 (2), 213-226
- https://doi.org/10.1016/0006-3002(61)90360-2
Abstract
A technique is described by which detailed information of the composition of an acidic polysaccharide mixture can be obtained. The polysaccharide-cetylpyridinium complex is precipitated on an inert support packed in a column, and is then eluted from the column in salt solutions of increasing concentration. The technique has been applied to the resolution of mixtures of known polysaccharides as well as to less well defined polysaccharide mixtures obtained from various tissues. A micromodification of the method allows as little as 25 μg of each polysaccharide component to be separated and determined.Keywords
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