Shear Stress Increases ICAM-1 and Decreases VCAM-1 and E-selectin Expressions Induced by Tumor Necrosis Factor-α in Endothelial Cells
- 1 January 2004
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Arteriosclerosis, Thrombosis, and Vascular Biology
- Vol. 24 (1), 73-79
- https://doi.org/10.1161/01.atv.0000106321.63667.24
Abstract
Objective—Vascular endothelial cells (ECs) are subjected to shear stress and cytokine stimulation. We studied the interplay between shear stress and cytokine in modulating the expression of adhesion molecule genes in ECs.Methods and Results—Shear stress (20 dynes/cm2) was applied to ECs prior to and/or following the addition of tumor necrosis factor (TNF)-α. Shear stress increased the TNF-α–induced expression of intercellular adhesion molecule-1 (ICAM-1) at both mRNA and surface protein levels, but decreased the TNF-α–induced expression of vascular adhesion molecule-1 (VCAM-1) and E-selectin. Transfection studies using promoter reporter gene constructs of ICAM-1, VCAM-1, and E-selectin demonstrated that these shear stress modulations of gene expression occur at the transcriptional levels. After 24-hour preshearing followed by 1 hour of static incubation, the effect of preshearing on TNF-α–induced ICAM-1 mRNA expression vanished. The recovery of the TNF-α–induced VCAM-1 and E-selectin mRNA expressions following preshearing, however, required a static incubation time of >6 hours (complete recovery at 24 hours). Pre- and postshearing caused a reduction in the nuclear factor-κB-DNA binding activity induced by TNF-α in the EC nucleus.Conclusions—Our findings suggest that shear stress plays differential roles in modulating the TNF-α–induced expressions of ICAM-1 versus VCAM-1 and E-selectin genes in ECs.Keywords
This publication has 19 references indexed in Scilit:
- Homocysteine Inhibits TNF-α-Induced Endothelial Adhesion Molecule Expression and Monocyte Adhesion via Nuclear Factor-κB Dependent PathwayBiochemical and Biophysical Research Communications, 2001
- Mitochondrial glutathione modulates TNF-α-induced endothelial cell dysfunctionFree Radical Biology & Medicine, 1999
- Shear stress inhibits apoptosis of human endothelial cellsFEBS Letters, 1996
- Reactive Oxygen Species Mediate Cytokine Activation of c-Jun NH2-terminal KinasesPublished by Elsevier BV ,1996
- Nitric oxide decreases cytokine-induced endothelial activation. Nitric oxide selectively reduces endothelial expression of adhesion molecules and proinflammatory cytokines.JCI Insight, 1995
- Stimulation of Transcription Factors NFκB and AP1 in Endothelial Cells Subjected to Shear StressBiochemical and Biophysical Research Communications, 1994
- Function and Activation of NF-kappaB in the Immune SystemAnnual Review of Immunology, 1994
- The pathogenesis of atherosclerosis: a perspective for the 1990sNature, 1993
- Functional analysis of the human vascular cell adhesion molecule 1 promoter.The Journal of Experimental Medicine, 1992
- Pulsatile flow and atherosclerosis in the human carotid bifurcation. Positive correlation between plaque location and low oscillating shear stress.Arteriosclerosis: An Official Journal of the American Heart Association, Inc., 1985