Production of extracellular polysaccharides by submerged mycelial culture of Laetiporus sulphureus var. miniatus and their insulinotropic properties
- 1 March 2008
- journal article
- biotechnological products-and-process-engineering
- Published by Springer Science and Business Media LLC in Applied Microbiology and Biotechnology
- Vol. 78 (3), 419-429
- https://doi.org/10.1007/s00253-007-1329-6
Abstract
In the present study, optimum culture conditions for the production of extracellular polysaccharides (EPS) in submerged culture of an edible mushroom, Laetiporus sulphureus var. miniatus and their stimulatory effects on insulinoma cell (RINm5F) proliferation and insulin secretion were investigated. The maximum mycelial growth (4.1 g l−1) and EPS production (0.6 g l−1) in submerged flask culture were achieved in a medium containing 30 g l−1 maltose, 2 g l−1 soy peptone, and 2 mM MnSO4·5H2O at an initial pH 2.0 and temperature 25°C. In the stirred-tank fermenter under optimized medium, the concentrations of mycelial biomass and EPS reached a maximum level of 8.1 and 3.9 g l−1, respectively. Interestingly, supplementation of deep sea water (DSW) into the culture medium significantly increased both mycelial biomass and EPS production by 4- and 6.7-fold at 70% (v/v) DSW medium, respectively. The EPS were proved to be glucose-rich polysaccharides and were able to increase proliferation and insulin secretary function of rat insulinoma RINm5F cells, in a dose-dependent manner. In addition, EPS also strikingly reduced the streptozotocin-induced apoptosis in RINm5F cells indicating the mode of the cytoprotective role of EPS on RINm5F cells.Keywords
This publication has 50 references indexed in Scilit:
- Effect of pH, temperature and nutrient limitations on growth and leukotoxin production by Mannheimia haemolytica in batch and continuous cultureJournal of Applied Microbiology, 2007
- Effects of ingested fruiting bodies, submerged culture biomass, and acidic polysaccharide glucuronoxylomannan of Tremella mesenterica Retz.:Fr. on glycemic responses in normal and diabetic ratsLife Sciences, 2006
- Anti-diabetic activity of β-glucans and their enzymatically hydrolyzed oligosaccharides from Agaricus blazeiBiotechnology Letters, 2005
- Hypoglycemic effect of crude exopolysaccharides produced by a medicinal mushroom Phellinus baumii in streptozotocin-induced diabetic ratsLife Sciences, 2005
- Optimization of submerged culture condition for the production of mycelial biomass and exopolysaccharides by Agrocybe cylindraceaBioresource Technology, 2004
- Effect of aeration rate on the mycelial morphology and exo-biopolymer production in Cordyceps militarisProcess Biochemistry, 2002
- Antidiabetic Effect of an Acidic Polysaccharide (TAP) from Tremella aurantia and Its Degradation Product (TAP-H).Biological & Pharmaceutical Bulletin, 2001
- Volatile composition of Laetiporus sulphureusCryptogamie Mycologie, 2000
- Effects of NaCI, Na2SO4, H2SO4, and glucose on growth, photosynthesis, and respiration in the acidophilic alga Dunaliella acidophila (Volvocales, Chlorophyta)Phycologia, 1988
- The Physiological Ecology of Cyanidium caldariumJournal of General Microbiology, 1971