The Major Allergen of the House Dust Mite, Dermatophagoides pteronyssinus, Is Synthesized and Secreted into Its Alimentary Canal
- 1 January 1988
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 85 (3), 312-315
- https://doi.org/10.1159/000234523
Abstract
The natural production of Der p1, the major faecal allergen from the house dust mite (Dermatophagoides pteronyssinus), was investigated. Mite cultures grown on radiolabelled substrate were examined at intervals over a period of 21 days and incorporation of radiolabel into the mite proteins measured over the time course. Mite proteins were extracted, purified by chromatofocussing and characterized by immunodiffusion, SDS-PAGE, electroblotting. and autoradiography. The faecal allergen, Der p1, did not incorporate a significant level of label until the 14th day (p < 0.05). The results suggest that Der p1 is a protein synthesized and excreted by the house dust mite.This publication has 2 references indexed in Scilit:
- The use of tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranesJournal of Immunological Methods, 1982
- Mite faeces are a major source of house dust allergensNature, 1981