A Role for Sphingomyelin-Rich Lipid Domains in the Accumulation of Phosphatidylinositol-4,5-Bisphosphate to the Cleavage Furrow during Cytokinesis
- 1 April 2012
- journal article
- Published by Taylor & Francis Ltd in Molecular and Cellular Biology
- Vol. 32 (8), 1396-1407
- https://doi.org/10.1128/mcb.06113-11
Abstract
Cytokinesis is a crucial step in the creation of two daughter cells by the formation and ingression of the cleavage furrow. Here, we show that sphingomyelin (SM), one of the major sphingolipids in mammalian cells, is required for the localization of phosphatidylinositol-4,5-bisphosphate (PIP(2)) to the cleavage furrow during cytokinesis. Real-time observation with a labeled SM-specific protein, lysenin, revealed that SM is concentrated in the outer leaflet of the furrow at the time of cytokinesis. Superresolution fluorescence microscopy analysis indicates a transbilayer colocalization between the SM-rich domains in the outer leaflet and PIP(2)-rich domains in the inner leaflet of the plasma membrane. The depletion of SM disperses PIP(2) and inhibits the recruitment of the small GTPase RhoA to the cleavage furrow, leading to abnormal cytokinesis. These results suggest that the formation of SM-rich domains is required for the accumulation of PIP(2) to the cleavage furrow, which is a prerequisite for the proper translocation of RhoA and the progression of cytokinesis.Keywords
This publication has 47 references indexed in Scilit:
- Evidence for a fence that impedes the diffusion of phosphatidylinositol 4,5-bisphosphate out of the forming phagosomes of macrophagesMolecular Biology of the Cell, 2011
- Inhibition of Glycosphingolipid Biosynthesis Induces Cytokinesis FailureJournal of the American Chemical Society, 2011
- Lipid Rafts As a Membrane-Organizing PrincipleScience, 2010
- A distinct pool of phosphatidylinositol 4,5-bisphosphate in caveolae revealed by a nanoscale labeling techniqueProceedings of the National Academy of Sciences of the United States of America, 2009
- Mechanisms for concentrating Rho1 during cytokinesisGenes & Development, 2009
- Photoactivatable mCherry for high-resolution two-color fluorescence microscopyNature Methods, 2009
- A Role for Very-Long-Chain Fatty Acids in Furrow Ingression during Cytokinesis in Drosophila SpermatocytesCurrent Biology, 2008
- Membrane traffic and polarization of lipid domains during cytokinesisBiochemical Society Transactions, 2008
- Male Germ Cells Require Polyenoic Sphingolipids with Complex Glycosylation for Completion of MeiosisOnline Journal of Public Health Informatics, 2008
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959