Characterization of Helper Virus-Independent Cytopathogenic Classical Swine Fever Virus Generated by an In Vivo RNA Recombination System
Open Access
- 15 February 2005
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 79 (4), 2440-2448
- https://doi.org/10.1128/jvi.79.4.2440-2448.2005
Abstract
Molecular analyses revealed that most cytopathogenic (cp) pestivirus strains evolve from noncytopathogenic (noncp) viruses by nonhomologous RNA recombination. In contrast to bovine viral diarrhea virus (BVDV), cp classical swine fever virus (CSFV) field isolates were rarely detected and always represented helper virus-dependent subgenomes. To investigate RNA recombination in more detail, we recently established an in vivo system allowing the efficient generation of recombinant cp BVDV strains in cell culture after transfecting a synthetic subgenomic and nonreplicatable transcript into cells being infected with noncp BVDV (A. Gallei, A. Pankraz, H.-J. Thiel, and P. Becher, J. Virol. 78: 6271-6281, 2004). Using an analogous approach, the first helper virus-independent cp CSFV strain (CP G1) has now been generated by RNA recombination. Accordingly, this study demonstrates the applicability of RNA recombination for designing new viral RNA genomes. The genomic RNA of CP G1 has a calculated size of 18.139 kb, almost 6 kb larger than all previously described CSFV genomes. It contains cellular sequences encoding a polyubiquitin fragment directly upstream of the nonstructural protein NS3 coding gene together with a duplication of viral sequences. CP G1 induces a cytopathic effect on different tissue culture cell lines from pigs and cattle. Subsequent analyses addressed growth kinetics, expression of NS3, and genetic stability of CP G1.Keywords
This publication has 34 references indexed in Scilit:
- Temporal Modulation of an Autoprotease Is Crucial for Replication and Pathogenicity of an RNA VirusJournal of Virology, 2004
- Virulence of Recent and Former Classical Swine Fever Virus Isolates Evaluated by their Clinical and Pathological SignsJournal of Veterinary Medicine, Series B, 2003
- Pathogenicity and kinetics of virus propagation in swine infected with the cytopathogenic classical swine fever virus containing defective interfering particlesArchiv für die gesamte Virusforschung, 2003
- Cellular Sequences in Pestivirus Genomes Encoding Gamma-Aminobutyric Acid (A) Receptor-Associated Protein and Golgi-Associated ATPase Enhancer of 16 KilodaltonsJournal of Virology, 2002
- RNA Recombination between Persisting Pestivirus and a Vaccine Strain: Generation of Cytopathogenic Virus and Induction of Lethal DiseaseJournal of Virology, 2001
- Characterization of Classical Swine Fever Virus Associated with Defective Interfering Particles Containing a Cytopathogenic Subgenomic RNA Isolated from Wild Boar.The Journal of Veterinary Medical Science, 2001
- Comparison of pestivirus multiplication in cells of different speciesResearch in Veterinary Science, 1994
- Ubiquitin in a togavirusNature, 1989
- Experimental production of fatal mucosal disease in cattleVeterinary Record, 1984
- Plaque production by Hog cholera virusArchiv für die gesamte Virusforschung, 1970