Mechanism of oligonucleotide uptake by cells: involvement of specific receptors?
- 1 September 1989
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 86 (17), 6454-6458
- https://doi.org/10.1073/pnas.86.17.6454
Abstract
We have investigated the interaction of oligonucleotides and their alkylating derivatives with mammalian cells. In experiments with L929 mouse fibroblast and Krebs 2 ascites carcinoma cells, it was found that cellular uptake of oligodeoxynucleotide derivatives is achieved by an endocytosis mechanism. Uptake is considerably more efficient at low oligomer concentration (< 1 .mu.M), because at this concentration a significant percentage of the total oligomer pool is absorbed on the cell surface and internalized by a more efficient absorptive endocytosis process. Two modified proteins were detected in mouse fibroblasts that were treated with the alkylating oligonucleotide derivatives. The binding of the oligomers to the proteins is inhibited by other oligodeoxynucleotides, single- and double-stranded DNA, and RNA. The polyanions heparin and chondroitin sulfates A and B do not inhibit binding. These observations suggest the involvement of specific receptor proteins in binding of oligomers to mammalian cells.This publication has 18 references indexed in Scilit:
- Characterization of oligonucleotide transport into living cells.Proceedings of the National Academy of Sciences of the United States of America, 1989
- Human promyelocytic leukemia HL-60 cell proliferation and c-myc protein expression are inhibited by an antisense pentadecadeoxynucleotide targeted against c-myc mRNA.Proceedings of the National Academy of Sciences of the United States of America, 1988
- Inhibition of replication and expression of human T-cell lymphotropic virus type III in cultured cells by exogenous synthetic oligonucleotides complementary to viral RNA.Proceedings of the National Academy of Sciences of the United States of America, 1986
- Evidence that pinocytosis in lymphoid cells has a low capacity.The Journal of cell biology, 1986
- Nucleotide and oligonucleotide derivatives as enzyme and nucleic acid targeted irreversible inhibitors, biochemical aspectsAdvances in Enzyme Regulation, 1985
- Nucleotide and oligonucleotide derivatives as enzyme and nucleic acid targeted irreversible inhibitors. Chemical aspectsAdvances in Enzyme Regulation, 1985
- Quantification of endocytosis-derived membrane trafficBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1985
- Complementary-Addressed (Sequence-Specific) Modification of Nucleic AcidsProgress in Nucleic Acid Research and Molecular Biology, 1985
- [18] Harvesting the products of cell growthMethods in Enzymology, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970