Immune Complexes in Transitional Cell Carcinoma
- 1 April 1980
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Journal of Urology
- Vol. 123 (4), 486-488
- https://doi.org/10.1016/s0022-5347(17)55983-2
Abstract
Bladder cancer patients from 2 different medical centers were examined to establish whether such patients have circulating immune complexes. Four methods of analysis were used: 1) polyethylene glycol precipitation, 2) double crossed immunoelectrophoresis, 3) Raji cell and 4) Clq binding assays. In the first group of 24 patients 17 had positive results on cystoscopy for pathologically defined tumors at the time of the serum sample. Two tested positive for the presence of circulating immune complexes by all 4 techniques and an additional 1 tested positive by 3 of the 4 techniques. In the second group of 54 patients (41 of whom had pathologically definable tumors at sample date) 9 were judged possibly positive by the Raji cell assay, the polyethylene glycol and double crossed immunoelectrophoresis techniques. When tested by the Clq binding assay 8 of the 9 patients were positive, most being in the range of 260 to 320 microgram/ml. immune complex. Combining all data from the 78 patients with bladder cancer the results in 10 cases definitely were positive by all 4 techniques and an additional 2 were positive by 3 techniques. Our data indicate that a low percentage (13 to 15 per cent) of patients with bladder cancer has circulating immune complexes. Of interest is that the complexes, as judged by our assay procedures, bind Clq, contain aggregated IgG and can be dissociated into antigen and antibody. Thus, the immune complexes are similar to those found in immune complex diseases.Keywords
This publication has 14 references indexed in Scilit:
- The C1q Binding Test for Soluble Immune Complexes: Clinical Correlations Obtained in Patients With Cancer 2JNCI Journal of the National Cancer Institute, 1977
- The Raji cell radioimmune assay for detecting immune complexes in human sera.JCI Insight, 1976
- Circulating immune complexes in schistosomiasis due to Schistosoma mansoni.BMJ, 1975
- Antibody Induction of Lymphocyte-Mediated Cytotoxicity against Human Transitional-Cell Carcinomas of the Urinary TractNew England Journal of Medicine, 1974
- Serum Induced Lymphoid Cell Mediated Cytotoxicity to Human Transitional Cell Carcinomas of the Genitourinary TractScience, 1974
- Lymphocyte-Mediated Cytotoxicity And Blocking Serum Activity To Tumor AntigensAdvances in Immunology, 1974
- Cellular immunity to human urinary bladder carcinoma. II. Effect of surgery and preoperative irradiationInternational Journal of Cancer, 1972
- Cellular immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapyInternational Journal of Cancer, 1972
- Elution of “blocking factors” from human tumors, capable of abrogating tumor‐cell destruction by specifically immune lymphocytesInternational Journal of Cancer, 1972
- Cellular and humoral immune responses to human urinary bladder carcinomasInternational Journal of Cancer, 1970