Degradation of Amyloid Proteins by Different Serine Proteases

Abstract

A protein extract was obtained from normal human serum by adsorption to unsubstituted Sepharose 4B. This extract contained one or several enzymes with SAA and AA degrading capacity. The optimal pH for degradation of SAA was about 7.3. On fractionation of the enzyme extract on Sephadex G-160, the active component was eluted in the V0 peak. The V0 fraction, which on double immunodiffusion analysis was found to contain alpha 2-macroglobulin, was also active against synthetic substrates used to determine the activity of thrombin and plasma kallikrein. Gel filtration under dissociating conditions and molecular weight estimation further indicated the presence of those enzymes in the preparation. Several serine proteases which are known to be inhibited by alpha 2-macroglobulin possessed AA and SAA degrading activity. On degradation of SAA, an intermediate split product with molecular weight similar to AA was formed. Kallikrein, plasmin and elastase were also able to degrade intact amyloid fibrils suspended in phosphate-buffered saline.