Inhibitory effect of oral Lactobacillus against oral pathogens
Open Access
- 31 July 2011
- journal article
- research article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 53 (4), 452-459
- https://doi.org/10.1111/j.1472-765x.2011.03132.x
Abstract
Aims: To determine the inhibitory effect of oral Lactobacillus against putative oral pathogens. Methods and Results: Total 357 strains comprising 10 species of oral Lactobacillus, Lactobacillus fermentum (195), Lactobacillus salivarius (53), Lactobacillus casei (20), Lactobacillus gasseri (18), Lactobacillus rhamnosus (14), Lactobacillus paracasei (12), Lactobacillus mucosae (12), Lactobacillus oris (12), Lactobacillus plantarum (11) and Lactobacillus vaginalis (10) were used as producer strains. Inhibitory effect against a panel of indicators, periodontitis- and caries-related pathogens, was assessed. Most oral Lactobacillus was able to inhibit the growth of both periodontitis- and caries-related pathogens. The strongest inhibitory activity was associated with Lact. paracasei, Lact. plantarum, Lact. rhamnosus, Lact. casei and Lact. salivarius. Lactobacillus SD1–SD6, representing the six species with the strong inhibitory effect, inhibited growth of Streptococcus mutans ATCC 25175 in the biofilm model. Also, it was demonstrated that growth of Strep. mutans was inhibited in a mixture with Lact. paracasei SD1. The inhibition was enhanced in acidic condition and 5% glucose. Conclusions: The results have shown that oral Lactobacillus SD1–SD6 showed a strong inhibitory effect against Strep. mutans and Streptococcus sobrinus, as well as, Gram-negative periodontal pathogens Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. Significance and Impact of the Study: The results indicated that Lactobacillus may be of benefit as probiotics for the prevention of oral diseases.Keywords
This publication has 26 references indexed in Scilit:
- Lactobacillusspecies and genotypes associated with dental caries in Thai preschool childrenMolecular Oral Microbiology, 2010
- Probiotics and periodontal diseasePeriodontology 2000, 2009
- Improvement of periodontal condition by probiotics with Lactobacillus salivarius WB21: a randomized, double‐blind, placebo‐controlled studyJournal of Clinical Periodontology, 2008
- Purification of bacteriocin LS1 produced by human oral isolate Lactobacillus salivarius BGHO1Oral Microbiology and Immunology, 2008
- Use of polymerase chain reaction techniques and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis for differentiation of oral Lactobacillus speciesOral Microbiology and Immunology, 2006
- Effect of Oral Lactobacillus salivarius TI2711 (LS1)Administration on Periodontopathic Bacteria in Subgingival Plaque.Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 2006
- Oral lactobacilli in chronic periodontitis and periodontal health: species composition and antimicrobial activityOral Microbiology and Immunology, 2005
- Suppression of Periodontal Pathogenic Bacteria in the Saliva of Humans by the Administration of Lactobacillus salivarius TI2711.Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 2003
- Production and sensitivity of bacteriocin-like activity among Porphyromonas gingivalis, Prevotella intermedia and Pr. nigrescens strains isolated from periodontal sitesJournal of Medical Microbiology, 1998
- Effect of pH on the Growth and Proteolytic Activity of Porphyromonas gingivalis and Bacteroides intermediusJournal of Dental Research, 1990