Dephosphorylation of human p34 cdc2 kinase on both Thr-14 and Tyr-15 by human cdc25B phosphatase
- 8 March 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 318 (3), 331-334
- https://doi.org/10.1016/0014-5793(93)80540-b
Abstract
In mammalian cells, p34 cdc2 kinase undergoes phosphorylation at threonine-14, tyrosine-15 and threonine-161 in the S and G2 phases of the cell cycle. At the onset of mitosis, the kinase becomes dephosphorylated at threonine-14 and tyrosine-15, resulting in activation. Cdc25 phosphatase has been shown to dephosphorylate tyrosine-15 in vitro, but whether it also does at threonine-14 remains unclear. In this study, we have found that human cdc25B phosphatase dephosphorylates both threonine-14 and tyrosine-15 but not threonine-161.Keywords
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