Mutation of Gluconobacter oxydans and Bacillus megaterium in a two-step process of l-ascorbic acid manufacture by ion beam
Open Access
- 8 April 2004
- journal article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 96 (6), 1317-1323
- https://doi.org/10.1111/j.1365-2672.2004.02270.x
Abstract
Aim: To increase the transformation rate of l-sorbose to 2-keto-l-gulonic (2-KLG) acid in a two-step process of l-ascrobic acid manufacture by ion beam. Methods and Results: Gluconobacter oxydans (GO29) and Bacillus megaterium (BM80) were used in the present study. Ion implantation was carried out with the heavy ion implantation facility at the institute of Plasma Physics in China. 2-KLG in whole culture broth was determined by iodometry. Mutants were screened by single-colony isolation and 2-KLG accumulation in broth. GO29 and BM80 were implanted by either hydrogen ions (H+) or nitrogen ions (N+) with various doses, respectively. The average transformation rate of GM112-302 bred by ion beam in Gram-molecule was increased from 79·3 to 94·5% after eight passages in shaking flasks. Furthermore, in 180-ton fermentors in Jiangsu Jiangshan Pharmaceutical Co. Ltd, the transformation rate was stable at 92·0%, indicating a producer could get 0·99 kg of gulonic acid from 1·0 kg of sorbose. Conclusion: Ion beam as a new mutation source had potential advantages in breeding. Comparing with original mixture GO29 and BM80, GM112-302 is more efficient in accumulating 2-KLG, especially at the later phase. Significance and Impact of the Study: GM112-302 bred by ion beam implantation dramatically increased the transformation rate by 19·2%, which greatly increased efficiency and reduced the cost of l-ascorbic acid manufacture in a two-step process.Keywords
This publication has 13 references indexed in Scilit:
- Biochemistry and biotechnological applications of Gluconobacter strainsApplied Microbiology and Biotechnology, 2002
- Ion-beam-induced deoxyribose nucleic acid transferApplied Physics Letters, 2001
- A study of low-energy ion beam effects on outer plant cell structure for exogenous macromolecule transferringSurface and Coatings Technology, 2000
- Reactions of solid glycine induced by keV ion irradiationChemical Physics, 1998
- Direct fermentation of 2‐keto‐l‐gulonic acid in recombinant Gluconobacter oxydansBiotechnology & Bioengineering, 1998
- Mass deposition in tyrosine irradiated by a N+ ionRadiation Physics and Chemistry, 1997
- Dose-effect of the tyrosine sample implanted by a low energy N+ ion beamRadiation Physics and Chemistry, 1994
- Microbial processes for ascorbic acid biosynthesis: a reviewEnzyme and Microbial Technology, 1990
- New mechanisms for the biosynthesis and metabolism of 2‐keto‐L‐gulonic acid in bacteriaBiotechnology & Bioengineering, 1975
- 2-Keto-L-gulonic Acid FermentationAgricultural and Biological Chemistry, 1968