Ultrastructural alterations and growth inhibition of Trypanosoma cruzi and Leishmania major induced by Bothrops jararaca venom

Abstract

Snake venom can affect the growth of Trypanosoma cruzi and Leishmania spp. As new classes of therapeutic drugs against protozoan parasites could be derived from snake venom, alterations in the ultrastructure and growth of the epimastigotes, trypomastigotes and amastigotes of T. cruzi, as well as the promastigotes of Leishmania major, were analyzed after treatment with crude venom from Bothrops jararaca. Parasite growth (epimastigotes and promastigotes) of venom treated cultures showed a negative correlation between cell growth and venom concentration. No growth occurred at a dose of 100 µg/ml of venom, while 50% growth inhibition was obtained in the range 0.1–0.3 µg/ml. Ultrastructural observations of treated bloodstream trypomastigotes, intracellular amastigotes, as well as axenic cultures of epimastigotes and promastigotes, demonstrated mitochondrial swelling and kinetoplast disorganization. Our data show that B. jararaca venom effectively inhibited the growth of T. cruzi and L. major parasites. Growth inhibition was probably related to mitochondrial impairment.