Transcriptome analysis of Taenia solium cysticerci using Open Reading Frame ESTs (ORESTES)
Open Access
- 31 July 2009
- journal article
- Published by Springer Science and Business Media LLC in Parasites & Vectors
- Vol. 2 (1), 35
- https://doi.org/10.1186/1756-3305-2-35
Abstract
Human infection by the pork tapeworm Taenia solium affects more than 50 million people worldwide, particularly in underdeveloped and developing countries. Cysticercosis which arises from larval encystation can be life threatening and difficult to treat. Here, we investigate for the first time the transcriptome of the clinically relevant cysticerci larval form. Using Expressed Sequence Tags (ESTs) produced by the ORESTES method, a total of 1,520 high quality ESTs were generated from 20 ORESTES cDNA mini-libraries and its analysis revealed fragments of genes with promising applications including 51 ESTs matching antigens previously described in other species, as well as 113 sequences representing proteins with potential extracellular localization, with obvious applications for immune-diagnosis or vaccine development. The set of sequences described here will contribute to deciphering the expression profile of this important parasite and will be informative for the genome assembly and annotation, as well as for studies of intra- and inter-specific sequence variability. Genes of interest for developing new diagnostic and therapeutic tools are described and discussed.Keywords
This publication has 61 references indexed in Scilit:
- Biomphalaria glabrata transcriptome: Identification of cell-signalling, transcriptional control and immune-related genes from open reading frame expressed sequence tags (ORESTES)Developmental & Comparative Immunology, 2007
- Identification of unannotated exons of low abundance transcripts in Drosophila melanogaster and cloning of a new serine protease gene upregulated upon injuryBMC Genomics, 2007
- A quantitative view of the transcriptome of Schistosoma mansoni adult-worms using SAGEBMC Genomics, 2007
- WoLF PSORT: protein localization predictorNucleic Acids Research, 2007
- Evaluation of recombinant HP6-Tsag, an 18 kDa Taenia saginata oncospheral adhesion protein, for the diagnosis of cysticercosisZeitschrift für Parasitenkunde, 2007
- InterPro and InterProScanMethods in molecular biology (Clifton, N.J.), 2007
- Molecular and functional characterization of a Taenia adhesion gene family (TAF) encoding potential protective antigens of Taenia saginata oncospheresZeitschrift für Parasitenkunde, 2006
- The structure and regulation of vinculinTrends in Cell Biology, 2006
- Effective Protection Against Experimental Taenia solium Tapeworm Infection in Hamsters by Primo-Infection and by Vaccination with Recombinant or Synthetic Heterologous AntigensJournal of Parasitology, 2006
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997