Metagenomic analysis of the pinewood nematode microbiome reveals a symbiotic relationship critical for xenobiotics degradation
Open Access
- 22 May 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 3 (1), 1869
- https://doi.org/10.1038/srep01869
Abstract
Our recent research revealed that pinewood nematode (PWN) possesses few genes encoding enzymes for degrading α-pinene, which is the main compound in pine resin. In this study, we examined the role of PWN microbiome in xenobiotics detoxification by metagenomic and bacteria culture analyses. Functional annotation of metagenomes illustrated that benzoate degradation and its related metabolisms may provide the main metabolic pathways for xenobiotics detoxification in the microbiome, which is obviously different from that in PWN that uses cytochrome P450 metabolism as the main pathway for detoxification. The metabolic pathway of degrading α-pinene is complete in microbiome, but incomplete in PWN genome. Experimental analysis demonstrated that most of tested cultivable bacteria can not only survive the stress of 0.4% α-pinene, but also utilize α-pinene as carbon source for their growth. Our results indicate that PWN and its microbiome have established a potentially mutualistic symbiotic relationship with complementary pathways in detoxification metabolism.This publication has 57 references indexed in Scilit:
- Comparative Genomic Analysis of the Endosymbionts of Herbivorous Insects Reveals Eco-Environmental Adaptations: Biotechnology ApplicationsPLoS Genetics, 2013
- Draft Genome Sequence of Serratia sp. Strain M24T3, Isolated from Pinewood Disease Nematode Bursaphelenchus xylophilusJournal of Bacteriology, 2012
- Pathogenicity of Aseptic Bursaphelenchus xylophilusPLOS ONE, 2012
- Integrative analysis of environmental sequences using MEGAN4Genome Research, 2011
- Computational and phylogenetic validation of nematode horizontal gene transferBMC Biology, 2011
- Diversity of Bacteria Associated with Bursaphelenchus xylophilus and Other Nematodes Isolated from Pinus pinaster Trees with Pine Wilt DiseasePLOS ONE, 2010
- De novo assembly of human genomes with massively parallel short read sequencingGenome Research, 2009
- Metagenome analysis of an extreme microbial symbiosis reveals eurythermal adaptation and metabolic flexibilityProceedings of the National Academy of Sciences of the United States of America, 2008
- Symbiotic conversations are revealed under genetic interrogationNature Reviews Microbiology, 2008
- MetaGene: prokaryotic gene finding from environmental genome shotgun sequencesNucleic Acids Research, 2006