Proteomic analysis of grapevine (Vitis vinifera L.) tissues subjected to herbicide stress
Open Access
- 10 October 2005
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 56 (421), 2783-2795
- https://doi.org/10.1093/jxb/eri271
Abstract
Two-dimensional gel electrophoresis coupled to mass spectrometry analysis was used to examine for the first time the effect of a herbicide (flumioxazin) on a crop species (Vitis vinifera L.) at the proteome level. Examination of 2-D maps derived from chemically stressed tissues revealed the presence of 33 spots displaying a differential expression pattern. The presence of stress responsive proteins in the different plant organs analysed suggests that flumioxazin could act systemically. Among the responsive proteins, some photosynthesis-related proteins, including several fragments of the enzyme Rubisco, were identified. This effect suggests that photosynthesis could be impaired by the herbicide. The induction of several enzymatic antioxidant systems was also observed, probably as a result of an oxidative stress. Moreover, the photorespiration pathway was stimulated, as suggested by the induction of some key enzymes involved in this process. Changes in carbon metabolism-associated proteins presumably reflect altered patterns of carbon flux in response to impaired photosynthesis and an increased need for osmotic adjustment in affected tissues. Finally, plant defences were stimulated as revealed by the induction of a set of proteins belonging to the pathogenesis-related 10 class, suggesting that they could play an essential role in cell defence mechanisms against flumioxazin.Keywords
This publication has 41 references indexed in Scilit:
- Plant PeroxiredoxinsAnnual Review of Plant Biology, 2003
- Crystal Structures of Two Homologous Pathogenesis-related Proteins from Yellow LupineJournal of Molecular Biology, 2002
- Drought differentially affects expression of a PR‐10 protein, in needles of maritime pine (Pinus pinaster Ait.) seedlingsJournal of Experimental Botany, 2001
- Patterns of Protein Synthesis and Tolerance of Anoxia in Root Tips of Maize Seedlings Acclimated to a Low-Oxygen Environment, and Identification of Proteins by Mass SpectrometryPlant Physiology, 2000
- Direct evidence for ribonucleolytic activity of a PR-10-like protein from white lupin rootsPlant Molecular Biology, 2000
- Water-deficit-responsive proteins in maritime pinePlant Molecular Biology, 1998
- The grand design of photosynthesis: Acclimation of the photosynthetic apparatus to environmental cuesPhotosynthesis Research, 1995
- Light intensity regulation of cab gene transcription is signaled by the redox state of the plastoquinone pool.Proceedings of the National Academy of Sciences, 1995
- Ascorbate peroxidase – a hydrogen peroxide‐scavenging enzyme in plantsPhysiologia Plantarum, 1992
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976