The Effects of Vadocaine, Dextromethorphan, Diphenhydramine and Hydroxyzine on the Ciliary Beat Frequency in Rats in Vitro
- 1 August 1990
- journal article
- research article
- Published by Wiley in Basic & Clinical Pharmacology & Toxicology
- Vol. 67 (2), 159-161
- https://doi.org/10.1111/j.1600-0773.1990.tb00804.x
Abstract
Mucociliary function is a major cleansing mechanism of the respiratory tract. Many drugs used in the treatment of respiratory diseases impair the ciliary beat frequency (CBF) of mucous membrane. Our aim was to study by means of a photoelectric technique, the effects of two antitussives - dextromethorphan and vadocaine - and two antihistamines -hydroxyzine and diphenhydramine - on the rat tracheal CBF in vitro. The CBF was measured from tracheal explants immersed in drug solutions. Dextromethorphan (1.0 mg/ml and 10.0 mg/ml) caused 16.9–20.8% decrease in the CBF during the 40 min. measurement period. Vadocaine (0.1 mg/ml and 0.5 mg/ml) decreased the CBF by 6.9%. Higher vadocaine concentrations caused a dose-dependent inhibitory effect so that mucociliary function stopped totally within 20 min. with 5.0 mg/ml vadocaine solution. Both diphenhydramine and hydroxyzine totally stopped the ciliary activity during 20 min. with concentrations of 2.5 mg/ml and 1.0 mg/ml, respectively. Locke-Ringer solution used as a control did not cause any change in the CBF. These results suggest that the antihistamines diphenhydramine and hydroxyzine are more ciliostatic than the antitussives dextromethorphan and vadocaine on the rat tracheal cilia in vitro. The results suggest further in vivo studies. The used photoelectric detection method proved to be suitable for evaluating drug effects on the CBF of respiratory mucosa.This publication has 6 references indexed in Scilit:
- Immotile cilia syndrome in childrenInternational Journal of Pediatric Otorhinolaryngology, 1983
- Adverse effects of lidocaine and methylparaben on tracheal ciliary activityThe Laryngoscope, 1979
- The Effect of Mucolytic Agents and Topical Decongestants on the Ciliary Activity of Chicken Tracheal Organ CulturesPediatric Research, 1977
- Antitussive activity of diphenhydramine in chronic coughClinical Pharmacology & Therapeutics, 1976
- Factors Affecting Ciliary Beat Frequency in the Intrapulmonary Airways of RatsCanadian Journal of Physiology and Pharmacology, 1975
- A Method for Standardized Studies of Mucociliary ActivityActa Oto-Laryngologica, 1974