In situ growth of hydroxyapatite within electrospun poly(DL‐lactide) fibers

Abstract

Development of nanocomposites of hydroxyapatite (HA) and polylactic acid (PLA) is attractive, as the advantageous properties of the two types of materials can be combined to suit better the mechanical and biological demands for biomedical uses. To solve the problematic issue of agglomeration of HA crystallites in the PLA matrix, a novel method is introduced in the present study to use electrospun nanofibers as the reaction confinement for composite fabrication. Poly(DL‐lactide) ultrafine fibers with calcium nitrate entrapment were prepared by electrospinning and then incubated in phosphate solution to form in situ calcium phosphate on the polymer matrix. The formation of nonstoichiometric nanostructured HA and well dispersion of HA particles on the electrospun fibers were observed. Higher crystalline HA phase was indicated in samples after sintering at 1200°C. The formation of the calcium‐phosphate phase was dependent upon the precipitation conditions, and the effects of the incubation time, temperature, and the pH values of the incubation medium were investigated on the spontaneous precipitation and amorphous‐crystalline transformation of HA in the current study. Considering the biodegradability of matrix polymer and the crystallinity and uniform dispersal of HA, optimal conditions for composite preparation were incubating calcium‐containing ultrafine fibers at 37°C in pH 7.4 or at 25°C in pH 9.0 of diammonium hydrogen phosphate solutions for 7 days. Around 25%–34% of mineral contents can be synthesized in the resulting composites, which was higher than the theoretical value due to the nonstoichiometric HA formed in the composite, and the fiber degradation and partial calcium nitrate involved in the HA formation. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007