Expression of TLR4-MyD88 and NF-B in the Iris during Endotoxin-Induced Uveitis
Open Access
- 1 January 2010
- journal article
- research article
- Published by Hindawi Limited in Mediators of Inflammation
- Vol. 2010, 1-7
- https://doi.org/10.1155/2010/748218
Abstract
Purpose. To observe the expression of Toll-like receptor-4 (TLR4), myeloid differentiation factor 88 (MyD88), and nuclear factor kappa B p65 (NF-B p65) in iris tissue during endotoxin-induced uveitis (EIU) and evaluate the significance of these factors in uveitis.Methods. Wistar rats were randomly divided into 5 groups (0 h, 12 h, 24 h, 48 h, and 72 h, n = 10/group). Animal model of acute anterior uveitis was established by a hind footpad injection of 200 Cholera vibrio LPS. Expression of TLR4, MyD88, and NF-B p65 in iris ciliary body tissue was detected through immunohistochemical staining.Results. Expression of TLR4 was not detected in normal iris-ciliary body complex, TLR4 positive cells with round morphology appeared in the iris stroma 12 hours after injection, significantly increased48 hours after injection, and decreased gradually 72 hours after injection. Expression of MyD88 and NF-B p65 is consistent with the change of the TLR4.Conclusions. The increased expression of TLR4 and its downstream signal transduction moleculesMyD88, NF-B p65 indicate the potential role of pathway in the pathogenesis of acute anterior uveitis (AAU).
Keywords
Funding Information
- National Natural Science Foundation of China (30872361, 2007BAI18B10)
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