A study of the canine gastric action potential in the presence of tetraethylammonium chloride.
- 1 April 1978
- journal article
- research article
- Published by Wiley in The Journal of Physiology
- Vol. 277 (1), 91-102
- https://doi.org/10.1113/jphysiol.1978.sp012262
Abstract
The effects of tetraethylammonium (TEA) ion on the action potential of isolated longitudinal muscle of the dog antrum were used to gain some insight into the mechanism of generation of the plateau potential of the action potential complex. The double sucrose gap was used. In concentrations of TEA up to 5 mM, the amplitude of the upstroke potential was increased. In 10 mM-TEA there was also an increase in the amplitude of the plateau potential and in the maximum rate of rise of the upstroke potential. Concentrations of TEA (3 mM and greater) increased the duration of the action potential. Five mM-TEA produced spike potentials which occurred only during the plateau potential of the action potential. Each spike caused a contraction. The steady-state voltage-current relation was studied in normal Krebs solution and in TEA containing Krebs solution. In normal Krebs solution the voltage response was not a linear function of the applied current when outward current pulses were used. In TEA solution the voltage response was a linear function of the entire range of applied depolarizing current. In low concentrations of TEA (2-4 mM), when the steady-state voltage-current relation was linear, constant current pulses were applied between action potentials and during the plateau potential to determine if there were a decrease in membrane slope resistance during the plateau. The amplitude of the electrotonic potential recorded during the plateau was significantly less than the amplitude of the electrotonic potential recorded between action potentials. The rate of repolarization of the plateau potential was studied in normal Krebs solution and in 2 mM-TEA Krebs solution. The rate of repolarization of the plateau potential was slowed in TEA Krebs solution. There is an increase in the membrane conductance during the plateau potential. The repolarization following the plateau potential is apparently due to a TEA-sensitive outward current.This publication has 17 references indexed in Scilit:
- The electrical basis of excitation and inhibition in the rat anoccygeus muscle.The Journal of Physiology, 1975
- Chemicals as tools in the study of excitable membranes.Physiological Reviews, 1974
- A study of pace‐maker activity in intestinal smooth muscleThe Journal of Physiology, 1974
- The Inner Quaternary Ammonium Ion Receptor in Potassium Channels of the Node of RanvierThe Journal of general physiology, 1972
- On the nature of the oscillations of the membrane potential (slow waves) produced by acetylcholine or carbachol in intestinal smooth muscleThe Journal of Physiology, 1971
- Effects of tetraethylammonium chloride on the membrane activity of guinea‐pig stomach smooth muscleThe Journal of Physiology, 1970
- THE MEMBRANE PROPERTIES AND DECREMENTAL CONDUCTION OF EXCITATION IN THE FUNDUS OF THE GUINEA-PIG STOMACHThe Japanese Journal of Physiology, 1970
- Effect of Tetraethyl Ammonium Ion on the Electrical Activity of Smooth Muscle CellNature, 1963
- The effect of quaternary ammonium ions on crustacean nerve fibresThe Journal of Physiology, 1953
- The electrical properties of crustacean muscle fibresThe Journal of Physiology, 1953