Graphene Oxide Sheet-Mediated Silver Enhancement for Application to Electrochemical Biosensors
- 22 December 2010
- journal article
- editorial
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 83 (3), 648-653
- https://doi.org/10.1021/ac103047c
Abstract
Functionalized graphene oxide (GO) sheets coupled with a signal amplification method based on the nanomaterial-promoted reduction of silver ions for the sensitive and selective detection of bacteria. This paper aims to develop an electrochemical route combined with GO sheet-mediated Ag enhancement for biological/chemical analyte detection. A linear relationship between the stripping response and the logarithm of the bacterial concentration was obtained using an electrochemical technique for concentrations ranging from 1.8 × 102 to 1.8 × 108 cfu mL−1, with a slope of 15.28 and a correlation coefficient of 0.995. Dot blot assay was used as a conventional immunoassay method for comparison with the electrochemical method, as well as to observe the quality of the anti-sulfate-reducing bacteria (SRB) antibody (Ab) used in the immunosensor. The GO sheet-mediated silver enhancement holds great potential for the rapid analysis of protein, DNA, and pathogens.Keywords
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