Measurement Area and Repeatability of Semiautomated Assessment of Corneal Endothelium in the Topcon Specular Microscope SP-2000P and IMAGEnet System
- 1 October 2012
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Cornea
- Vol. 31 (10), 1111-1118
- https://doi.org/10.1097/ico.0b013e31820e42b5
Abstract
To investigate the repeatability of the semiautomatic assessment of corneal endothelial cells and its association with the measurement area in the Topcon SP-2000P microscope and IMAGEnet system. Specular microscopic images of 86 healthy subjects were captured and analyzed using the Topcon SP-2000P microscope and IMAGEnet system. The same images were analyzed twice, on separate days, by the same examiner using the built-in measurement tool of the IMAGEnet system. The measurement areas were defined with a frame mounted on a computer screen. Four different-sized measurement areas were chosen for the semiautomatic measurements: box A (5.4 × 13.9 cm(2)), box B (4 × 10 cm(2)), box C (4 × 7 cm(2)), and box D (2 × 5 cm(2)). Average cell size (ACS), endothelial cell density (ECD), coefficient of variance, and hexagonality were measured. Repeatability was assessed based on the limit of agreement (LOA). The means of ACS, ECD, and hexagonality were not statistically different across 4 measurement areas (analysis of variance, P > 0.05). The mean differences (bias) were modest for ACS (range, -1.9∼3.9 μm(2)), ECD (range, -27.2∼14.6 cells per square millimeter), coefficient of variance (range, -0.14∼1.00), and hexagonality (range, -1.3%∼6.8%). Limits of agreement (mean difference ± 1.96× SD) were greater in the measurements with smaller areas: limit of agreement values for ECD were 14.6 ± 99.6, -3.8 ± 101.1, -27.2 ± 179, and -15.8 ± 488 cells per square millimeter for boxes A, B, C, and D, respectively. Similar trends were found in the repeatability of ACS and hexagonality. Repeatability is improved when larger measurement areas are chosen.Keywords
This publication has 20 references indexed in Scilit:
- Changes in Corneal Endothelial Cells after Ahmed Glaucoma Valve Implantation: 2-Year Follow-upAmerican Journal of Ophthalmology, 2009
- Endothelial cell density after photorefractive keratectomy for moderate myopia using a 213 nm solid-state laser systemJournal of Cataract & Refractive Surgery, 2007
- SAFETY PROFILE OF A TAURINE CONTAINING IRRIGATION SOLUTION (AcriProTect) IN PARS PLANA VITRECTOMYRetina, 2007
- The Effect of Contact Lens Wear on the Central and Peripheral Corneal EndotheliumCornea, 2000
- In Vivo Confocal Microscopy of Fuchsʼ Endothelial DystrophyCornea, 1998
- The Effect of Photorefractive Keratectomy on the Corneal EndotheliumOphthalmology, 1996
- Corneal endothelial cell loss in phacoemulsification surgery with silicone intraocular lens implantationJournal of Cataract & Refractive Surgery, 1996
- Wide-Field Endothelial Counting PanelsAmerican Journal of Ophthalmology, 1985
- Central Corneal Endothelial Cell Density After Argon Laser TrabeculoplastyAmerican Journal of Ophthalmology, 1984
- Swelling of the Cornea in vivo after the Destruction of its Limiting LayersBritish Journal of Ophthalmology, 1951