Bone consolidation is enhanced by rhBMP‐2 in a rabbit model of distraction osteogenesis
- 1 July 2002
- journal article
- Published by Wiley in Journal of Orthopaedic Research
- Vol. 20 (4), 779-788
- https://doi.org/10.1016/s0736-0266(01)00166-8
Abstract
Recombinant human bone morphogenetic protein-2 (rhBMP-2) is a differentiation factor which has been shown to induce bone formation and heal bony defects in a variety of animal models. A possible application of rhBMP-2 is to accelerate bone regeneration during distraction osteogenesis. which clinically is a long procedure, often involving significant complications. In this study we tested the ability of rhBMP-2 to accelerate the consolidation phase of distraction osteogenesis in a rabbit model of leg lengthening. Tibiae were lengthened 2 cm over a period of ten days. rhBMP-2 was administered at the end of the lengthening phase. Two modes of rhBMP-2 application were tested: surgical implantation of rhBMP-2/ACS (absorbable collagen sponge) into the regenerate (50 microl of 1.5 mg/ml rhBMP-2, total dose = 75 microg rhBMP-2), and percutaneous injection of rhBMP-2/buffer (0.1 ml of 0.75 mg/ml rhBMP-2. total dose = 75 microg rhBMP-2) into three sites within the regenerate. Also, there were three groups of control animals: (1) no surgical intervention, (2) surgical implantation of buffer/ACS and (3) percutaneous injection of buffer. Rabbits were sacrificed at 5, 14 and 28 days after the interventions. Radiographic evaluation indicated a significant increase in bony union of the distraction regenerate in the rhBMP-2 treated groups compared with the untreated groups at 5 and 14 days. At 28 days, formation of a cortex and reestablishment of the medullary canal was evident only in the rhBMP-2 treated groups. The bone mineral content (BMC) of the regenerate was significantly higher in the rhBMP-2 treated groups at 5 and 14 days. However, at 28 days, BMC of the regenerate was similar in all groups. The average volumetric density of the regenerate was significantly higher in the rhBMP-2 injection group at day 14. In Summary, both injection of rhBMP-2/buffer and implantation of rhBMP-2/ACS enhanced the consolidation stage of distraction osteogenesis in this rabbit model.Keywords
This publication has 34 references indexed in Scilit:
- Evaluation of recombinant human bone morphogenetic protein‐2 as a bone‐graft substitute in a canine segmental defect modelJournal of Orthopaedic Research, 2000
- Involvement of ERK in BMP-2 Induced Osteoblastic Differentiation of Mesenchymal Progenitor Cell Line C3H10T1/2Biochemical and Biophysical Research Communications, 2000
- Biologic model of bone transport distraction osteogenesis and vascular responseJournal of Orthopaedic Research, 1999
- Bone Regeneration and Fracture HealingPublished by Ovid Technologies (Wolters Kluwer Health) ,1998
- The bone morphogenetic protein family and osteogenesisMolecular Reproduction and Development, 1992
- Recombinant human bone morphogenetic protein-2 stimulates osteoblastic maturation and inhibits myogenic differentiation in vitro.The Journal of cell biology, 1991
- Leg LengtheningClinical Orthopaedics and Related Research, 1989
- The Histology of Distraction Osteogenesis Using Different External FixatorsPublished by Ovid Technologies (Wolters Kluwer Health) ,1989
- The Tension-Stress Effect on the Genesis and Growth of TissuesPublished by Ovid Technologies (Wolters Kluwer Health) ,1989
- The Tension-Stress Effect on the Genesis and Growth of TissuesPublished by Ovid Technologies (Wolters Kluwer Health) ,1989