IL7‐hCD25 and IL7‐Cre BAC transgenic mouse lines: New tools for analysis of IL‐7 expressing cells
- 4 March 2009
- Vol. 47 (4), 281-287
- https://doi.org/10.1002/dvg.20497
Abstract
IL-7 is a cytokine that is required for T-cell development and homeostasis as well as for lymph node organogenesis. Despite the importance of IL-7 in the immune system and its potential therapeutic relevance, questions remain regarding the sites of IL-7 synthesis, specific cell types involved and molecular mechanisms regulating IL-7 expression. To address these issues, we generated two bacterial artificial chromosome (BAC) transgenic mouse lines in which IL-7 regulatory elements drive expression of either Cre recombinase or a human CD25 (hCD25) cell surface reporter molecule. Expression of the IL-7.hCD25 BAC transgene, detected by reactivity with anti-hCD25 antibody, mimicked endogenous IL-7 expression. Fetal and adult tissues from crosses between IL-7.Cre transgenic mice and Rosa26R or R26-EYFP reporters demonstrated X-gal or YFP staining in tissues known to express endogenous IL-7 at some stage during development. These transgenic lines provide novel genetic tools to identify IL-7 producing cells in various tissues and to manipulate gene expression selectively in IL-7 expressing cells. genesis 47:281–287, 2009.Keywords
This publication has 28 references indexed in Scilit:
- 'Coreceptor tuning': cytokine signals transcriptionally tailor CD8 coreceptor expression to the self-specificity of the TCRNature Immunology, 2007
- Notch–RBP-J signaling controls the homeostasis of CD8− dendritic cells in the spleenThe Journal of Experimental Medicine, 2007
- IL-7–induced proliferation of recent thymic emigrants requires activation of the PI3K pathwayBlood, 2006
- Role of T and NK cells and IL7/IL7r interactions during neonatal maturation of lymph nodesProceedings of the National Academy of Sciences, 2006
- Regulation of mature T cell homeostasisSeminars in Immunology, 2005
- Lymphocide: cytokines and the control of lymphoid homeostasisNature Reviews Immunology, 2002
- Bac to the future: The use of bac transgenic mice for neuroscience researchNature Reviews Neuroscience, 2001
- Bcl-2 Rescues T Lymphopoiesis in Interleukin-7 Receptor–Deficient MiceCell, 1997
- Early lymphocyte expansion is severely impaired in interleukin 7 receptor-deficient mice.The Journal of Experimental Medicine, 1994
- Interleukin-7: a Cofactor For V(D)J Rearrangement of the T Cell Receptor β GeneScience, 1993