Nitric Oxide–Dependent Suppression of Thioredoxin-Interacting Protein Expression Enhances Thioredoxin Activity
- 1 December 2006
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Arteriosclerosis, Thrombosis, and Vascular Biology
- Vol. 26 (12), 2666-2672
- https://doi.org/10.1161/01.atv.0000248914.21018.f1
Abstract
Objective— Cellular redox balance is regulated by enzymatic and nonenzymatic systems and freely diffusible nitric oxide (NO) promotes antioxidative mechanisms. We show the NO-dependent transcriptional regulation of the antioxidative thioredoxin system. Methods and Results— Incubation of rat pulmonary artery smooth muscle cells (RPaSMC) with the NO donor compound S -nitroso-glutathione (GSNO, 100 μmol/L) suppressed thioredoxin-interacting protein (Txnip), an inhibitor of thioredoxin function, by 71±18% and enhanced thioredoxin reductase 2.7±0.2 fold (n=6; both P P cis -regulatory elements between −1777 and −1127 bp upstream of the start codon. Hyperglycemia induced Txnip promoter activity (3.9±0.2-fold; P P Conclusions— NO can regulate cellular redox state by changing expression of Txnip and thioredoxin reductase. This represents a novel antioxidative mechanism of NO independent of posttranslational protein S -nitrosylation of thioredoxin.Keywords
This publication has 28 references indexed in Scilit:
- Importin α1 (Rch1) Mediates Nuclear Translocation of Thioredoxin-binding Protein-2/Vitamin D3-up-regulated Protein 1Published by Elsevier BV ,2004
- Hyperglycemia Promotes Oxidative Stress through Inhibition of Thioredoxin Function by Thioredoxin-interacting ProteinPublished by Elsevier BV ,2004
- Redox regulatory and anti-apoptotic functions of thioredoxin depend on S-nitrosylation at cysteine 69Nature, 2002
- Cyclic nucleotide research — still expanding after half a centuryNature Reviews Molecular Cell Biology, 2002
- Vitamin D3-up-regulated Protein-1 Is a Stress-responsive Gene That Regulates Cardiomyocyte Viability through Interaction with ThioredoxinPublished by Elsevier BV ,2002
- Overexpression of VDUP1 mRNA sensitizes HeLa cells to paraquatBiochemical and Biophysical Research Communications, 2002
- NitrosylationCell, 2001
- A Possible Interaction of Thioredoxin with VDUP1 in HeLa Cells Detected in a Yeast Two-Hybrid SystemBiochemical and Biophysical Research Communications, 2000
- Distinct Roles of Thioredoxin in the Cytoplasm and in the NucleusJournal of Biological Chemistry, 1999
- Nitric oxide decreases stability of mRNAs encoding soluble guanylate cyclase subunits in rat pulmonary artery smooth muscle cells.JCI Insight, 1997