Binding of prostaglandin to human serum albumin
- 1 June 1972
- journal article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 24 (6), 470-477
- https://doi.org/10.1111/j.2042-7158.1972.tb09034.x
Abstract
The interaction of tritiated prostaglandin E1 (3H-PGE1) with various constituents of human blood was examined by equilibrium dialysis and incubation experiments. Plasma bound 3H-PGE1 weakly, apparently due to an interaction with serum albumin. Blood cells and plasma globulins did not interact with PG. When albumin and other proteins were precipitated by ethanol, as a preliminary stage of extracting PG from plasma, the recoveries of added PGs were poor. Addition of acid and non-precipitating amounts of ethanol (40–50%) to the plasma allowed quantitative extraction of added PGE, F, and A compounds into chloroform.Keywords
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