Genetic Requirements for Inheritance of RNAi in C. elegans
- 31 March 2000
- journal article
- editorial
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 287 (5462), 2494-2497
- https://doi.org/10.1126/science.287.5462.2494
Abstract
In Caenorhabditis elegans , the introduction of double-stranded RNA triggers sequence-specific genetic interference (RNAi) that is transmitted to offspring. The inheritance properties associated with this phenomenon were examined. Transmission of the interference effect occurred through a dominant extragenic agent. The wild-type activities of the RNAi pathway genes rde-1 and rde-4 were required for the formation of this interfering agent but were not needed for interference thereafter. In contrast, the rde-2 and mut-7 genes were required downstream for interference. These findings provide evidence for germ line transmission of an extragenic sequence-specific silencing factor and implicate rde-1 and rde-4 in the formation of the inherited agent.Keywords
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