Rh2, a compound extracted from ginseng, hypersensitizes multidrug-resistant tumor cells to chemotherapy
- 1 July 2004
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Physiology and Pharmacology
- Vol. 82 (7), 431-437
- https://doi.org/10.1139/y04-049
Abstract
Rh2 is a ginsenoside extracted from ginseng that has drawn attention in a few laboratories in Asian countries because of its potential tumor-inhibitory effect. In the present study, we tested Rh2 on many tumor-cell lines for its effects on cell proliferation, induction of apoptosis, and potential interaction with conventional chemotherapy agents. Our results showed that Rh2 inhibited cell growth by G1 arrest at low concentrations and induced apoptosis at high concentrations in a variety of tumor-cell lines, possibly through activation of caspases. The growth arrest and apoptosis may be mediated by 2 separate mechanisms. Apoptosis is not dependent on expression of the wild-type p53 nor the caspase 3. In addition, the apoptosis induced by Rh2 was mediated through glucocorticoid receptors. Most interestingly, Rh2 can act either additively or synergistically with chemotherapy drugs on cancer cells. Particularly, it hypersensitized multidrug-resistant breast cancer cells to paclitaxel. These results suggest that Rh2 possesses strong tumor-inhibiting properties, and potentially can be used in treatments for multidrug-resistant cancers, especially when it is used in combination with conventional chemotherapy agents.Key words: ginsenosides, synergy, paclitaxel, chemotherapy, cancer.Keywords
This publication has 15 references indexed in Scilit:
- American ginseng and breast cancer therapeutic agents synergistically inhibit MCF-7 breast cancer cell growthJournal of Surgical Oncology, 1999
- Update from Asia: Asian Studies on Cancer ChemopreventionAnnals of the New York Academy of Sciences, 1999
- Inhibition of Human Caspases by Peptide-based and Macromolecular InhibitorsJournal of Biological Chemistry, 1998
- Inhibitory Effects of Ginsenoside Rh2 on Tumor Growth in Nude Mice Bearing Human Ovarian Cancer CellsJapanese Journal of Cancer Research, 1998
- Caspase-3 Is Required for DNA Fragmentation and Morphological Changes Associated with ApoptosisOnline Journal of Public Health Informatics, 1998
- Resistance of MCF7 human breast carcinoma cells to TNF-induced cell death is associated with loss of p53 functionOncogene, 1997
- Ginsenoside-Rh2 blocks the cell cycle of SK-HEP-1 cells at the G1/S boundary by selectively inducing the protein expression of p27kip1Cancer Letters, 1996
- Reversal of Daunomycin and Vinblastine Resistance in Multidrug-Resistant P388 Leukemiain vitrothrough Enhanced Cytotoxicity by TriterpenoidsPlanta Medica, 1995
- Interactions of Ginseng Extract, Ginseng Separated Fractions, and Some Triterepenoid Saponins with Glucose Transporters in Sheep ErythrocytesPlanta Medica, 1994
- Inhibition of human ovarian cancer cell proliferation in vitro by ginsenoside Rh2 and adjuvant effects to cisplatin in vivoAnti-Cancer Drugs, 1991