miR-21 and miR-155 are associated with mitotic activity and lesion depth of borderline melanocytic lesions
Open Access
- 23 August 2011
- journal article
- research article
- Published by Springer Science and Business Media LLC in British Journal of Cancer
- Vol. 105 (7), 1023-1029
- https://doi.org/10.1038/bjc.2011.288
Abstract
Expression of microRNAs (miRs) has been shown to be altered in many solid tumours and is being explored in melanoma. The malignant potential of some melanocytic lesions is difficult to predict. We hypothesised that characterisation of miR expression in borderline melanocytic proliferations would lead to the identification of a molecular profile that could be used with known prognostic factors to differentiate lesions with high malignant potential. The miR expression profile of melanocytic lesions (benign naevi, malignant melanoma and borderline melanocytic tumours) was evaluated by real-time PCR. PCR analysis revealed primary cutaneous melanomas had an 8.6-fold overexpression of miR-21 and a 7.5-fold overexpression of miR-155 compared with benign naevi (PIn situ hybridisation confirmed these results. miR-21 and miR-155 were significantly overexpressed within borderline lesions (P=0.0011 and P=0.0048, respectively). When borderline lesions were categorised by mitotic activity and Breslow thickness, miR-21 was associated with mitotic activity and miR-155 was associated with thickness (P<0.025). Among 14 patients with borderline lesions who underwent sentinel lymph node biopsy (SLNB), positive SLNB was associated with increased miR-21 and miR-155 in the primary lesion compared with lesions with a negative SLNB. MicroRNA expression profiles can be used to characterise atypical melanocytic lesions.Keywords
This publication has 42 references indexed in Scilit:
- Modulation of K-Ras-Dependent Lung Tumorigenesis by MicroRNA-21Cancer Cell, 2010
- Pre-B cell proliferation and lymphoblastic leukemia/high-grade lymphoma in Eμ-miR155 transgenic miceProceedings of the National Academy of Sciences, 2006
- Unique microRNA molecular profiles in lung cancer diagnosis and prognosisCancer Cell, 2006
- A microRNA expression signature of human solid tumors defines cancer gene targetsProceedings of the National Academy of Sciences, 2006
- Lack of BIC and microRNA miR‐155 expression in primary cases of Burkitt lymphomaGenes, Chromosomes and Cancer, 2005
- REporting recommendations for tumour MARKer prognostic studies (REMARK)European Journal Of Cancer, 2005
- MicroRNA-21 Is an Antiapoptotic Factor in Human Glioblastoma CellsCancer Research, 2005
- MicroRNA expression profiles classify human cancersNature, 2005
- Spitz nevi and atypical Spitz nevi/tumors: a histologic and immunohistochemical analysisLaboratory Investigation, 2005
- Prognostic Factors Analysis of 17,600 Melanoma Patients: Validation of the American Joint Committee on Cancer Melanoma Staging SystemJournal of Clinical Oncology, 2001