Antisera against an acetylcholine receptor α3 fusion protein bind to ganglionic but not to brain nicotinic acetylcholine receptors
- 6 November 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 257 (2), 393-399
- https://doi.org/10.1016/0014-5793(89)81580-7
Abstract
Neuronal nicotinic acetylcholine receptor (AChR) subtypes have been defined pharmacologically, immunologically, and by DNA cloning, but the correlations between these approaches are incomplete. Vertebrate neuronal AChRs that have been isolated are composed of structural subunits and ACh-binding subunits. A single kind of subunit can be used in more than one AChR subtype. Monoclonal antibody (mAb) 35 binds to structural subunits of subtypes of AChRs from both chicken brain and ganglia. By using antisera to a unique sequence of α3 ACh-binding subunits expressed in bacteria, we show that ganglionic AChRs contain α3 ACh-binding subunits, whereas the brain AChR subtype that binds mAb 35 does not. Subunit-specific antisera raised against recombinant proteins should be a valuable approach for identifying the subunit composition of receptors in multigene, multisubunit familiesKeywords
This publication has 46 references indexed in Scilit:
- Three-dimensional structure of the nicotinic acetylcholine receptor and location of the major associated 43-kD cytoskeletal protein, determined at 22 A by low dose electron microscopy and x-ray diffraction to 12.5 A [published erratum appears in J Cell Biol 1989 Oct;109(4 Pt 1):1185]The Journal of cell biology, 1989
- Functional chloride channels by mammalian cell expression of rat glycine receptor subunitNeuron, 1989
- Cloning of a Putative Neuronal Nicotinic Acetylcholine Receptor SubunitJournal of Neurochemistry, 1989
- Expression and regulation of neuronal acetylcholine receptor mRNA in chick ciliary gangliaNeuron, 1988
- cDNA clones coding for the structural subunit of a chicken brain nicotinic acetylcholine receptorNeuron, 1988
- Neuronal nicotinic acetylcholine receptor β‐subunit is coded for by the cDNA clone α4FEBS Letters, 1987
- Affinity labelling of neuronal acetylcholine receptors localizes acetylcholine-binding sites to their β-subunitsFEBS Letters, 1987
- T7 RNA polymerase can direct expression of influenza virus cap-binding protein (PB2) in Escherichia coliGene, 1987
- Ganglionic nAChRs and high‐affinity nicotinic binding sites are not equivalentFEBS Letters, 1986
- Molecular distinction between fetal and adult forms of muscle acetylcholine receptorNature, 1986