Induction of apoptosis in human lymphoblastoid cells by kaempferol 3-O-β-isorhamninoside and rhamnocitrin 3-O-β-isorhamninoside from Rhamnus alaternus L. (Rhamnaceae)
- 28 April 2011
- journal article
- Published by Wiley in Cell Proliferation
- Vol. 44 (3), 283-290
- https://doi.org/10.1111/j.1365-2184.2011.00749.x
Abstract
Kaempferol 3-O-β-isorhamninoside (K3O-ir) and rhamnocitrin 3-O-β-isorhamninoside (R3O-ir) from Rhamnus alaternus L leaves are investigated for their ability to induce apoptosis in human lymphoblastoid cells. We have attempted to characterize apoptotic pathway activated by these two flavonoids. Apoptosis of the human TK6 lymphoblastoid cell line was detected by DNA fragmentation, PARP cleavage and by evaluating caspase activity. Apoptosis was observed after 24- and 48-h incubation of the cells with the tested compounds. DNA fragmentation was observed after treatment with flavonoids; this was confirmed by demonstration of PARP cleavage. Caspase-3 and caspase-8 activities were induced by both K3O-ir and R3O-ir flavonoids showing highest activity with compound concentration of 400 μg/ml. We have demonstrated that K3O-ir and R3O-ir induce apoptosis in human lymphoblastoid cells by the extrinsic pathway of apoptosis.Keywords
This publication has 40 references indexed in Scilit:
- 2001 Warkany lecture: To die or not to die, the role of apoptosis in normal and abnormal mammalian developmentTeratology, 2002
- Cytotoxic Activity of Sesquiterpenoids from Atractylodes ovata on Leukemia Cell LinesPlanta Medica, 2002
- Poly (ADP-ribose) polymerase, nitric oxide and cell deathTrends in Pharmacological Sciences, 1999
- Cell Death in DevelopmentCell, 1999
- Molecular mechanisms in the antiproliferative action of quercetinLife Sciences, 1997
- Programmed Cell Death in Animal DevelopmentCell, 1997
- Protease activation during apoptosis: Death by a thousand cuts?Cell, 1995
- Apoptosis in the Pathogenesis and Treatment of DiseaseScience, 1995
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976