Use of Silica Gel with Aqueous Eluent for Simultaneous High Performance Liquid Chromatographic Assay of Disopyramide and Mono-N-Dealkyldisopyramide

Abstract

Summary: Disopyramide and its pharmacologically active metabolite, mono-N-dealkyldisopyramide, were determined simultaneously in serum by high performance liquid chromatography using a bare (unbonded) silica gel with aqueous eluents. p-Chlorodisopyramide was used as the internal standard. Separations of all compounds, which contain amine moiety in their chemical structures, were easily accomplished with a good peak symmetry on silica. The method is sufficiently precise, sensitive, and specific. Analytical recoveries of all compounds were >96%; CVs for reproducibility were N-dealkyldisopyramide; the lower detection limits of both analytes were 0.05 mg/L. Comparison of the present method with a fluorescence polarization immunoassay for disopyramide gives a good correlation (r = 0.992).