Lack of Association of Ghrelin Precursor Gene Variants and Percentage Body Fat or Serum Lipid Profiles
- 1 April 2008
- Vol. 16 (4), 908-912
- https://doi.org/10.1038/oby.2007.125
Abstract
Ghrelin has been recognized for its involvement in food intake, control of energy homeostasis, and lipid metabolism. However, the roles of genetic variations in the ghrelin precursor gene (GHRL) on body compositions and serum lipids are not clear in humans. Our study investigated five single-nucleotide polymorphisms (SNPs) within GHRL to determine their relationship with body fat percentage (BF), trunk fat percentage (TF), lower body (legs) fat percentage (LF), and serum lipids in 1,464 subjects, which were recruited from the genetically homogeneous population of Newfoundland and Labrador (NL), Canada. Serum glucose, insulin, total cholesterol, high-density lipoprotein-cholesterol, low-density lipoprotein-cholesterol, and triglycerides were determined. Five SNPs are rs35684 (A/G: a transition substitution in exon 1), rs4684677 (A/T: a missense mutation), rs2075356 (C/T: intron), rs26802 (G/T: intron), and rs26311 (A/G: near the 3' untranslated region) of GHRL were genotyped using TaqMan validated or functionally tested SNP genotyping assays. Our study found no significant evidence of an allele or genotype association between any of the variant sites and body compositions or serum lipids. Furthermore, haplotype frequencies were not found to be significantly different between lean and obese subjects. In summary, the results of our study do not support a significant role for genetic variations in GHRL in the differences of body fat and serum lipid profiles in the NL population.Keywords
This publication has 24 references indexed in Scilit:
- The Human Obesity Gene Map: The 2005 UpdateObesity, 2006
- Altered Calcium Homeostasis Is Correlated With Abnormalities of Fasting Serum Glucose, Insulin Resistance, and β-Cell Function in the Newfoundland PopulationDiabetes, 2005
- Detecting Disease Associations due to Linkage Disequilibrium Using Haplotype Tags: A Class of Tests and the Determinants of Statistical PowerHuman Heredity, 2003
- Ghrelin: Update 2003Published by Oxford University Press (OUP) ,2003
- Role of Ghrelin Polymorphisms in Obesity Based on Three Different StudiesObesity Research, 2002
- Ghrelin Gene: Identification of Missense Variants and a Frameshift Mutation in Extremely Obese Children and Adolescents and Healthy Normal Weight StudentsJournal of Clinical Endocrinology & Metabolism, 2002
- The Novel Hypothalamic Peptide Ghrelin Stimulates Food Intake and Growth Hormone SecretionEndocrinology, 2000
- R: A Language for Data Analysis and GraphicsJournal of Computational and Graphical Statistics, 1996
- A Receptor in Pituitary and Hypothalamus That Functions in Growth Hormone ReleaseScience, 1996
- Chi-Square Tests with One Degree of Freedom; Extensions of the Mantel- Haenszel ProcedureJournal of the American Statistical Association, 1963