Glycerol Monooleate-Based Nanocarriers for siRNA Delivery in Vitro
- 10 August 2012
- journal article
- research article
- Published by American Chemical Society (ACS) in Molecular Pharmaceutics
- Vol. 9 (9), 2450-2457
- https://doi.org/10.1021/mp200662f
Abstract
We present studies of the delivery of short interfering ribonucleic acid (siRNA) into a green fluorescent protein (GFP) expressing cell line, using lipid nanocarriers in cubic lyotropic liquid crystal form. These carriers are based on glycerol monooleate (GMO) and employ the use of varying concentrations of cationic siRNA binding lipids. The essential physicochemical parameters of the cationic lipid/GMO/siRNA complexes such as particle size, ζ otential, siRNA uptake stability, lyotropic mesophase behavior, cytotoxicity,and gene silencing efficiency were systematically assessed. We find that the lipid nanocarriers were effectively taken up by mammalian cells and that their siRNA payload was able to induce gene silencing in vitro. More importantly, it was found that the nonlamellar structure of some of the lipid nanocarrier formulations were more effective at gene silencing than their lamellar structured counterparts. The development of cationic lipid functionalized nonlamellar GMO-based nanostructured nanoparticles may lead to improved siRNA delivery vehicles.Keywords
This publication has 27 references indexed in Scilit:
- Self-Assembled Multicompartment Liquid Crystalline Lipid Carriers for Protein, Peptide, and Nucleic Acid Drug DeliveryAccounts of Chemical Research, 2010
- Highly Efficient Gene Silencing Activity of siRNA Embedded in a Nanostructured Gyroid Cubic Lipid MatrixJournal of the American Chemical Society, 2010
- A Novel Mechanism Is Involved in Cationic Lipid-Mediated Functional siRNA DeliveryMolecular Pharmaceutics, 2009
- Knocking down barriers: advances in siRNA deliveryNature Reviews Drug Discovery, 2009
- Modulation of a membrane lipid lamellar–nonlamellar phase transition by cationic lipids: A measure for transfection efficiencyBiochimica et Biophysica Acta (BBA) - Biomembranes, 2008
- A combinatorial library of lipid-like materials for delivery of RNAi therapeuticsNature Biotechnology, 2008
- Cubic membranes: a structure-based design for DNA uptakeJournal of The Royal Society Interface, 2008
- An intracellular lamellar–nonlamellar phase transition rationalizes the superior performance of some cationic lipid transfection agentsProceedings of the National Academy of Sciences of the United States of America, 2006
- Cubic membranes: a legend beyond the Flatland* of cell membrane organizationThe Journal of cell biology, 2006
- Potent and persistent in vivo anti-HBV activity of chemically modified siRNAsNature Biotechnology, 2005