Strong substrate-stabilizing effect of a water-miscible ionic liquid [BMIM][BF4] in the catalysis of horseradish peroxidase
- 1 November 2007
- journal article
- Published by Springer Science and Business Media LLC in Biotechnology Letters
- Vol. 30 (3), 529-533
- https://doi.org/10.1007/s10529-007-9570-8
Abstract
The effects of a water-miscible ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4]), on both thermodynamics and kinetic mechanism of the horseradish peroxidase (HRP)-catalyzed oxidation of guaiacol (2-methoxyphenol) by H2O2 were investigated. The ionic liquid stabilized the ground state of guaiacol by causing an 8-fold increase of Km from 3 to 23 mM upon the addition of 25% (v/v) [BMIM][BF4]. In addition, the effect of [BMIM][BF4] in decreasing the kcat value of HRP catalysis was described by a non-competitive inhibition mechanism. The value of the inhibition constant of [BMIM][BF4] was 2.9 M indicating that the ionic liquid plays the role of a weak non-competitive inhibitor for HRP catalysis.Keywords
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