Structure and mechanism of DNA topoisomerase II
Open Access
- 18 January 1996
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 379 (6562), 225-232
- https://doi.org/10.1038/379225a0
Abstract
The crystal structure of a large fragment of yeast type II DNA topoisomerase reveals a heart-shaped dimeric protein with a large central hole. It provides a molecular model of the enzyme as an ATP-modulated clamp with two sets of jaws at opposite ends, connected by multiple joints. An enzyme with bound DNA can admit a second DNA duplex through one set of jaws, transport it through the cleaved first duplex, and expel it through the other set of jaws.Keywords
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