Needle-Type Dual Microsensor for the Simultaneous Monitoring of Glucose and Insulin
- 19 January 2001
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 73 (4), 844-847
- https://doi.org/10.1021/ac0009393
Abstract
A miniature needle-type sensor suitable for the simultaneous amperometric monitoring of glucose and insulin is described. The integrated microsensor consists of dual (biologically and chemically) modified carbon-paste working electrodes inserted into a 14-guage needle. The glucose probe is based on the biocatalytic action of glucose oxidase, and the insulin one relies on the electrocatalytic activity of ruthenium oxide. The analytical performance of the dual sensor is assessed under flow injection conditions. The needle dual detector exhibits a very rapid response to dynamic changes in the concentrations of glucose and insulin. No apparent cross reactivity is observed in mixtures containing millimolar glucose levels and nanomolar insulin concentrations. The response is highly linear (to at least 1000 nM insulin and 14 mM glucose) and reproducible (RSD = 2.6−4.1%). The combination microsensor holds great promise for real-time measurements of the insulin/glucose ratio and for improved management of diabetes.Keywords
This publication has 9 references indexed in Scilit:
- Electroanalysis and BiosensorsAnalytical Chemistry, 1999
- An Integrated Needle-Type Biosensor for Intravascular Glucose and Lactate MonitoringElectroanalysis, 1998
- Ruthenium catalyst for amperometric determination of insulin at physiological pHJournal of Electroanalytical Chemistry, 1997
- Extracellular pH Is Required for Rapid Release of Insulin from Zn−Insulin Precipitates in β-Cell Secretory Vesicles during ExocytosisJournal of the American Chemical Society, 1996
- Highly Selective Membrane-Free, Mediator-Free Glucose BiosensorAnalytical Chemistry, 1994
- Amperometric monitoring of chemical secretions from individual pancreatic .beta.-cellsAnalytical Chemistry, 1993
- Serum insulin/glucose ratio and urinary loss of insulin after trauma in young animals: effect of adrenergic blockadePediatric Surgery International, 1993
- Flow injection amperometric determination of insulin based upon its oxidation at a modified electrodeAnalytical Chemistry, 1989
- Potentiometric combination ion-carbon dioxide sensors for in vitro and in vivo blood measurementsAnalytical Chemistry, 1989