Association of the Agrobacterium T-DNA–protein complex with plant nucleosomes
- 7 October 2008
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 105 (40), 15429-15434
- https://doi.org/10.1073/pnas.0805641105
Abstract
Agrobacterium represents the only natural example of transkingdom transfer of genetic information, from bacteria to plants. Before the bacterial transferred DNA (T-DNA) can integrate into the plant genome, it should be targeted to and bind the host chromatin. However, the T-DNA association with the host chromatin has not been demonstrated. Here, we study T-DNA binding to plant nucleosomes in vitro and show that it is mediated by bacterial and host proteins associated with the T-DNA. The main factor that determines nucleosomal binding of the T-DNA is the cellular VirE2-interacting protein 1 (VIP1), which functions as a molecular link between the T-DNA-associated bacterial virulence protein VirE2 and core histones. The presence of both VIP1 and VirE2 is required for association of the T-DNA with mononucleosomes in which the DNA molecule exists as a tripartite complex DNA-VirE2-VIP1. Furthermore, this nucleosome-associated ternary complex can bind another bacterial virulence factor, VirF, which is an F-box protein known to target both VirE2 and VIP1 for proteasomal degradation and uncoat the T-DNA.Keywords
This publication has 34 references indexed in Scilit:
- Chromatin Modifications and Their FunctionCell, 2007
- Identification and Characterization of Plant Genes Involved inAgrobacterium-Mediated Plant Transformation by Virus-Induced Gene SilencingMolecular Plant-Microbe Interactions®, 2007
- The Agrobacterium VirE3 effector protein: a potential plant transcriptional activatorNucleic Acids Research, 2006
- Biological systems of the host cell involved in Agrobacterium infectionCellular Microbiology, 2006
- Chromatin techniques for plant cellsThe Plant Journal, 2004
- Three-dimensional Reconstruction of Agrobacterium VirE2 Protein with Single-stranded DNAPublished by Elsevier BV ,2004
- Distribution and characterization of over 1000 T‐DNA tags in rice genomeThe Plant Journal, 2003
- Genome-Wide Insertional Mutagenesis of Arabidopsis thalianaScience, 2003
- Agrobacterium -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” ToolMicrobiology and Molecular Biology Reviews, 2003
- Initiation of Agrobacterium tumefaciens T-DNA ProcessingJournal of Biological Chemistry, 1995