Establishment of Cell Lines with High- and Low-metastatic Potential from PC-14 Human Lung Adenocarcinoma
- 1 February 2001
- journal article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 92 (2), 174-183
- https://doi.org/10.1111/j.1349-7006.2001.tb01080.x
Abstract
This article reports the establishment of variant cell lines with high and low metastatic potential by repeated selection and the dilution plating technique. Five clones with high metastatic potential, Lu‐2, Lu‐7, Lu‐4, Lu‐1 and Lu‐5, and four clones with low metastatic potential, 3S, 7S, 8S and 13S, were established from PC‐14 human lung adenocarcinoma. The high‐metastatic cell lines produced enhanced lung metastases, but the low‐metastatic cell lines did not produce lung metastasis by injection into the tail vein of 5‐week‐old BALB/c nude mice. The high‐metastatic cell lines produced enhanced tumors on both visceral and parietal pleurae, and enhanced metastases to the mediastinum and contralateral pleural cavity. The low‐metastatic cell lines produced reduced tumors on both visceral and parietal pleurae and reduced metastases to the mediastinum and contralateral pleural cavity after injection into the left preceral cavity of the nude mice. When the nine variant cell lines and original PC‐14 cells were embedded in collagen gels, the PC‐14 cells and the low‐metastatic cell lines gave rise to colonies with a dendritic morphology, and cells were tightly associated. The high‐metastatic cell lines were more loosely associated and scattered into three‐dimensional colonies. These nine cloned cell lines originated from heterogeneous populations of the parental PC‐14 cells should be useful tools for studying the process of metastasis of lung cancer.Keywords
This publication has 61 references indexed in Scilit:
- Isolation of a Novel Actin-Related Gene Expressed in Low-Metastatic PC-14 Human Lung AdenocarcinomaBiochemical and Biophysical Research Communications, 2001
- Absence of Germline CHK2 Mutations in Familial Gastric CancerJapanese Journal of Cancer Research, 2000
- Angiogenic potential of malignant and non-malignant human breast tissues in anin vivo angiogenesis modelInternational Journal of Cancer, 1998
- Angiogenesis and metastasisEuropean Journal of Cancer, 1996
- Metastasis from human breast cancer cell linesBreast Cancer Research and Treatment, 1996
- Significance of angiogenesis in tumour progression and metastasisEuropean Journal of Cancer, 1995
- A variant actin (βm) reduces metastasis of mouse B16 melanomaInternational Journal of Cancer, 1994
- Connective tissue growth factor: a cysteine-rich mitogen secreted by human vascular endothelial cells is related to the SRC-induced immediate early gene product CEF-10.The Journal of cell biology, 1991
- Evidence for the Clonal Origin of Spontaneous MetastasesScience, 1982
- The pathogenesis of cancer metastasisNature, 1980