Cell volume change through water efflux impacts cell stiffness and stem cell fate
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Open Access
- 25 September 2017
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 114 (41), 201705179-E8627
- https://doi.org/10.1073/pnas.1705179114
Abstract
Cells alter their mechanical properties in response to their local microenvironment; this plays a role in determining cell function and can even influence stem cell fate. Here, we identify a robust and unified relationship between cell stiffness and cell volume. As a cell spreads on a substrate, its volume decreases, while its stiffness concomitantly increases. We find that both cortical and cytoplasmic cell stiffness scale with volume for numerous perturbations, including varying substrate stiffness, cell spread area, and external osmotic pressure. The reduction of cell volume is a result of water efflux, which leads to a corresponding increase in intracellular molecular crowding. Furthermore, we find that changes in cell volume, and hence stiffness, alter stem-cell differentiation, regardless of the method by which these are induced. These observations reveal a surprising, previously unidentified relationship between cell stiffness and cell volume that strongly influences cell biology.Keywords
Funding Information
- HHS | National Institutes of Health (NIH) (P01GM096971)
- HHS | National Institutes of Health (NIH) (P01HL120839)
- National Science Foundation (NSF) (DMR-1420570)
- National Science Foundation (NSF) (DMR-1310266)
- HHS | National Institutes of Health (NIH) (HL86655)
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