Application of immunoassays for the herbicides, molinate and diuron, to field water analysis
- 1 September 1996
- journal article
- research article
- Published by Taylor & Francis Ltd in Food and Agricultural Immunology
- Vol. 8 (3), 169-183
- https://doi.org/10.1080/09540109609354915
Abstract
Immunoassays based on microwells (for laboratory assay) and polystyrene tubes (for field assay) have been applied to the analysis of the herbicides, molinate and diuron, in field water samples. Development of a new immunoassay format for molinate, with detection limits of 0.5–1 ppb, enabled, for the first time, the direct analysis of molinate in water samples across the full range of usual field residue levels, without need for pre‐concen‐tration. A highly sensitive field assay for urea herbicides (diuron limit of detection of 0.15 ppb) has also been developed, based on a double‐layer coating with protein A and urea herbicide‐specific antibody. Each assay was only slightly affected by ions and turbidity, which are often problems in subsurface and surface water matrices respectively. For both formats of the molinate and diuron immunoassays, close correlations were obtained between herbicide levels determined by the immunoassay and by instrumental analysis, using field water samples.Keywords
This publication has 19 references indexed in Scilit:
- Development and Application of Laboratory and Field Immunoassays for Chlorpyrifos in Water and Soil MatrixesJournal of Agricultural and Food Chemistry, 1994
- Synthesis of Haptens and Derivation of Monoclonal Antibodies for Immunoassay of the Phenylurea Herbicide DiuronJournal of Agricultural and Food Chemistry, 1994
- Determination of the insect growth regulator methoprene in wheat grain and milling fractions using an enzyme immunoassayJournal of Agricultural and Food Chemistry, 1991
- The EC Directive on Drinking Water (EEC 80/778)Water and Environment Journal, 1989
- Evaluation of an enzyme‐linked immunosorbent assay (ELISA) for the direct analysis of molinate (Ordram®) in rice field waterFood and Agricultural Immunology, 1989
- Development of an enzyme-linked immunosorbent assay for the analysis of the thiocarbamate herbicide molinateJournal of Agricultural and Food Chemistry, 1988
- Bromacil and diuron herbicides: Toxicity, uptake, and elimination in freshwater fishArchives of Environmental Contamination and Toxicology, 1987
- Residues of diuron and phytotoxic degradation products in aquatic situations. II. Diuron in irrigation waterPesticide Science, 1978
- Persistence and Factors Affecting Dissipation of Molinate under Flooded Rice CultureJournal of Environmental Quality, 1978
- Conjugation of antibodies with fluorochromes: Modifications to the standard methodsJournal of Immunological Methods, 1976