Single step BP/LR combined Gateway reactions
Open Access
- 1 November 2013
- journal article
- Published by Future Science Ltd in BioTechniques
- Vol. 55 (5), 265-268
- https://doi.org/10.2144/000114101
Abstract
The Gateway recombination system is characterized by its ability to transfer DNA sequences back and forth between an intermediate clone (the entry clone) and a variety of destination vectors. However, a number of applications do not need to exploit the advantages offered by the entry clone. Here we report reaction conditions for cloning DNA fragments into destination vectors in a single step reaction, thus reducing the cost and overall time needed to obtain an expression clone from three days to one.Keywords
This publication has 4 references indexed in Scilit:
- Gateway®recombinational cloning: a biological operating systemExpert Opinion on Drug Discovery, 2007
- Concerted Assembly and Cloning of Multiple DNA Segments Using In Vitro Site-Specific Recombination: Functional Analysis of Multi-Segment Expression ClonesGenome Research, 2004
- DNA Cloning Using In Vitro Site-Specific RecombinationGenome Research, 2000
- DYNAMIC, STRUCTURAL, AND REGULATORY ASPECTS OF λ SITE-SPECIFIC RECOMBINATIONAnnual Review of Biochemistry, 1989