Optical Recognition of Converted DNA Nucleotides for Single-Molecule DNA Sequencing Using Nanopore Arrays
- 11 May 2010
- journal article
- letter
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 10 (6), 2237-2244
- https://doi.org/10.1021/nl1012147
Abstract
We demonstrate the feasibility of a nanopore based single-molecule DNA sequencing method, which employs multicolor readout. Target DNA is converted according to a binary code, which is recognized by molecular beacons with two types of fluorophores. Solid-state nanopores are then used to sequentially strip off the beacons, leading to a series of detectable photon bursts, at high speed. We show that signals from multiple nanopores can be detected simultaneously, allowing straightforward parallelization to large nanopore arrays.This publication has 20 references indexed in Scilit:
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