Analyses of brain tumor cell lines confirm a simple model of relationships among fluorescence in situ hybridization, DNA index, and comparative genomic hybridization
- 7 December 1997
- journal article
- research article
- Published by Wiley in Genes, Chromosomes and Cancer
- Vol. 20 (4), 311-319
- https://doi.org/10.1002/(sici)1098-2264(199712)20:4<311::aid-gcc1>3.0.co;2-4
Abstract
Several techniques are commonly used for genetic analysis of interphase nuclei. Flow cytometry assays the distribution of DNA content in populations of nuclei stained with a DNA‐specific fluorochrome. Fluorescence in situ hybridization (FISH) quantifies the number of copies of a specific DNA sequence in single nuclei. Comparative genomic hybridization (CGH) assesses the relative copy number of DNA sequences throughout a test genome by comparing the signal intensities of test and reference DNA samples hybridized to a template of normal metaphase chromosomes. In principle, there are specific relationships among data obtained from these measurements, and combined measurements should provide a more comprehensive view of the sample that is analyzed. We applied these three techniques to nine brain tumor cell lines and find that a model of CGH that includes unsuppressed repeat sequences describes the data well. We estimate that up to 35% of the fluorescence intensity in well‐blocked CGH preparations may not represent unique sequences. Taking these factors into account, our results are, in general, mutually consistent, and highlight issues critical for interpreting CGH preparations. Genes Chromosomes Cancer 20:311‐319, 1997.Keywords
This publication has 19 references indexed in Scilit:
- Mapping of chromosomal gains and losses in prostate cancer by comparative genomic hybridizationGenes, Chromosomes and Cancer, 1995
- Chromosomal abnormalities in glioblastoma multiforme tumors and glioma cell lines detected by comparative genomic hybridizationInternational Journal of Cancer, 1995
- Optimizing comparative genomic hybridization for analysis of DNA sequence copy number changes in solid tumorsGenes, Chromosomes and Cancer, 1994
- Detection and mapping of amplified DNA sequences in breast cancer by comparative genomic hybridization.Proceedings of the National Academy of Sciences, 1994
- Detection of complete and partial chromosome gains and losses by comparative genomic in situ hybridizationHuman Genetics, 1993
- Comparative Genomic Hybridization for Molecular Cytogenetic Analysis of Solid TumorsScience, 1992
- Detection of aneuploidy and aneuploidy-inducing agents in human lymphocytes using fluorescence in situ hybridization with chromosome-specific DNA probesMutation Research/Environmental Mutagenesis and Related Subjects, 1990
- DNA ploidy and survival in breast cancer patientsCytometry, 1987
- On the ratio of two correlated normal random variablesBiometrika, 1969
- THE DISTRIBUTION OF THE INDEX IN A NORMAL BIVARIATE POPULATIONBiometrika, 1932