Forkhead Box M1 (FoxM1) Gene Is a New STAT3 Transcriptional Factor Target and Is Essential for Proliferation, Survival and DNA Repair of K562 Cell Line
Open Access
- 24 October 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (10), e48160
- https://doi.org/10.1371/journal.pone.0048160
Abstract
The forkhead box (Fox) M1 gene belongs to a superfamily of evolutionarily conserved transcriptional regulators that are involved in a wide range of biological processes, and its deregulation has been implicated in cancer survival, proliferation and chemotherapy resistance. However, the role of FoxM1, the signaling involved in its activation and its role in leukemia are poorly known. Here, we demonstrate by gene promoter analysis, Electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) assays that FoxM1 is a new target of the STAT3 transcriptional activator. Additionally, FoxM1 is transcriptionally dependent on STAT3 signaling activation. Furthermore, we verified that FoxM1 is crucial for K562 cell proliferation, cell cycle checkpoints and viability and could be related to chemotherapeutic resistance. By microarray analysis, we determined the signaling pathways related to FoxM1 expression and its role in DNA repair using K562 cells. Our results revealed new signaling involved in FoxM1 expression and its role in leukemic cells that elucidate cellular mechanisms associated with the development of leukemia and disease progression.Keywords
This publication has 45 references indexed in Scilit:
- Integrated genomic analyses of ovarian carcinomaNature, 2011
- Activation of the hedgehog pathway in chronic myelogeneous leukemia patientsJournal of Experimental & Clinical Cancer Research, 2011
- FoxM1 Mediates Resistance to Herceptin and PaclitaxelCancer Research, 2010
- Upregulation of FOXM1 induces genomic instability in human epidermal keratinocytesMolecular Cancer, 2010
- Inhibition of matrix metalloproteinase‐2 enhances radiosensitivity by abrogating radiation‐induced FoxM1‐mediated G2/M arrest in A549 lung cancer cellsInternational Journal of Cancer, 2009
- Stat3 contributes to resistance toward BCR-ABL inhibitors in a bone marrow microenvironment model of drug resistanceMolecular Cancer Therapeutics, 2008
- Plk1-dependent phosphorylation of FoxM1 regulates a transcriptional programme required for mitotic progressionNature, 2008
- FoxM1 Regulates Transcription of JNK1 to Promote the G1/S Transition and Tumor Cell InvasivenessPublished by Elsevier BV ,2008
- Protocol for the fast chromatin immunoprecipitation (ChIP) methodNature Protocols, 2006
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001