Impact on fatty acid metabolism and differential localization of FATP1 and FAT/CD36 proteins delivered in cultured human muscle cells
- 1 June 2005
- journal article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 288 (6), C1264-C1272
- https://doi.org/10.1152/ajpcell.00271.2004
Abstract
We compared the intracellular distribution and regulatory role of fatty acid transporter protein (FATP1) and fatty acid translocase (FAT/CD36) on muscle cell fatty acid metabolism. With the use of adenoviruses, FATP1 and FAT genes were delivered to primary cultured human muscle cells. FATP1 and FAT moderately enhanced palmitate and oleate transport evenly at concentrations of 0.05, 0.5, and 1 mM. Long-term (16 h) consumption of palmitate and oleate from the media, and particularly incorporation into triacylglyceride (TAG), was stimulated equivalently by FATP1 and FAT at all fatty acid concentrations tested. In contrast, long-term CO2production was reduced by FATP1 and FAT at all doses of palmitate and at the lower concentrations of oleate. Neither FATP1 nor FAT markedly altered the production of acid-soluble metabolic intermediates from palmitate or oleate. The intracellular localization of fusion constructs of FATP1 and FAT with enhanced green fluorescent protein (EGFP) was examined. Independently of fatty acid treatment, FATPGFP was observed throughout the cytosol in a reticular pattern and concentrated in the perinuclear region, partly overlapping with the Golgi marker GM-130. FATGFP was found in the extracellular membrane and in cytosolic vesicles not coincident with GM-130. Neither FATP1 nor FAT proteins colocalized with lipid droplets in oleate-treated cells. We conclude that whereas FAT is localized on the extracellular membrane, FATP1 is active in the cytosol and imports fatty acids into myotubes. Overall, both FATP1 and FAT stimulated transport and consumption of palmitate and oleate, which they channeled away from complete oxidation and toward TAG synthesis.Keywords
This publication has 29 references indexed in Scilit:
- Endocytosis of Oxidized Low Density Lipoprotein through Scavenger Receptor CD36 Utilizes a Lipid Raft Pathway That Does Not Require Caveolin-1Published by Elsevier BV ,2003
- A common polymorphism in the fatty acid transport protein-1 gene associated with elevated post-prandial lipaemia and alterations in LDL particle size distributionAtherosclerosis, 2003
- FATP1 channels exogenous FA into 1,2,3-triacyl-sn-glycerol and down-regulates sphingomyelin and cholesterol metabolism in growing 293 cellsJournal of Lipid Research, 2002
- Insulin Causes Fatty Acid Transport Protein Translocation and Enhanced Fatty Acid Uptake in AdipocytesDevelopmental Cell, 2002
- Membrane Topology of the Murine Fatty Acid Transport Protein 1Published by Elsevier BV ,2001
- Palmitoylation of Caveolin-1 at a Single Site (Cys-156) Controls Its Coupling to the c-Src Tyrosine KinasePublished by Elsevier BV ,2001
- Defective Fatty Acid Uptake in the Spontaneously Hypertensive Rat Is a Primary Determinant of Altered Glucose Metabolism, Hyperinsulinemia, and Myocardial HypertrophyPublished by Elsevier BV ,2001
- Acute Regulation of Fatty Acid Uptake Involves the Cellular Redistribution of Fatty Acid TranslocasePublished by Elsevier BV ,2000
- The Fatty Acid Transport Protein (FATP1) Is a Very Long Chain Acyl-CoA SynthetaseJournal of Biological Chemistry, 1999
- 14CO2 production is no adequate measure of [14C]fatty acid oxidationBiochemical Medicine and Metabolic Biology, 1986