Tetrazine–trans-cyclooctene ligation for the rapid construction of 18F labeled probes
- 22 September 2010
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- Vol. 46 (42), 8043-8045
- https://doi.org/10.1039/c0cc03078c
Abstract
A radiolabeling method for bioconjugation based on the Diels–Alder reaction between 3,6-diaryl-s-tetrazines and an 18F-labeled trans-cyclooctene is described. The reaction proceeds with exceptionally fast rates, making it an effective conjugation method within seconds at low micromolar concentrations.Keywords
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