Dodecyl maltoside detergent improves resolution of hepatic membrane proteins in two‐dimensional gels

Abstract

This report describes the incorporation of an alkyl maltoside detergent in two‐dimensional polyacrylamide gel Electrophoresis (2‐D PAGE) sample lysis buffer in order to improve resolution of protein patterns separated by nonequilibrium pH gradient Electrophoresis. Membrane‐associated proteins with alkaline isoelectric points form horizontal streaks on two‐dimensional electrophoretograms when solubilized with conventional nonionic detergent. Dodecyl maltoside enhances protein delipidation during solubilization and improves pattern resolution and protein mobility.