CELL YIELDS OF VIBRIO MARINUS, AN OBLIGATE PSYCHROPHILE, AT LOW TEMPERATURE

Abstract

The cell yields and generation time for Vibrio marinus, an obligate psychrophile, were determined. Cell yields of 13 × 10¹¹and 9 × 10⁹cells/ml in 24 hours at 15 °C (organism"s optimum growth temperature) and 3 °C (organism"s natural environmental temperature) respectively were obtained. Besides temperature, the type of medium also influenced the cell yields. The generation time during the exponential growth phase was calculated to be 80.7 minutes at 15 °C and 226 minutes at 3 °C. Growth was obtained at various hydrostatic pressures (1 to 400 atm) at both 3 and 15 °C.